They accuse Doha of destabilizing the region through supporting proxies of Shiite Muslim Iran and the Sunni militants of al-Qaeda and Islamic State, charges Qatar has repeatedly denied.
For the US military, which is supporting Iraqi troops assaulting Mosul and readying a major push into Raqqa, the Islamic State's de facto capital in Syria, the base has never been more important.
Media captionThe economic disruption could have an impact on Qatar if the dispute drags on.
Qatari investigators have said that a cyber attack which saw the publication of explosive fake news last month that helped trigger a crisis with Gulf neighbours was prepared in April.
"There is no proof", Meshal bin Hamad Al Thani, Qatari ambassador to the USA, told Al Jazeera. "Terror financing is across the whole globe".
- Donald J. Trump (@realDonaldTrump) June 6, 2017So good to see the Saudi Arabia visit with the King and 50 countries already paying off.
It is the worst split between powerful Arab states in decades.
Referring to the regional group that includes Qatar, the President also "reiterated that a united Gulf Cooperation Council and a strong United States-Gulf Cooperation Council partnership are critical to defeating terrorism and promoting regional stability".
London Bridge attacker known to security services as two terrorists named
Butt was known to police and "openly fundamentalist", NPR's Eleanor Beardsley reports from London . As part of the bombing investigation 21 people have been arrested, of whom 12 are now free.
The Reuters news agency noted a tweet sent by Hamid Aboutalebi, deputy chief of staff of Iran's President Hassan Rouhani. "It is trying to describe this as an issue about the independence of their foreign policy, and it is not".
"He said every country in the region has their own obligations and they need to live up to terminate their support for terrorism and extremism however it manifests itself anywhere in the world", she said. The official is pointing to offers that Kuwait and Turkey have made to mediate. "But the US still wants to see this issue de-escalated and resolved immediately, keeping with the principles that the president laid out in terms of defeating terror financing and extremism", he said.
Saudi Arabia and the UAE used to be the biggest markets by the number of available seats for Qatar Airways.
Mr. Trump had said on Twitter that during his trip to the Middle East, he warned about funding "radical ideology" and leaders there had "pointed to Qatar".
As a member of the Saudi-led Arab coalition, Qatar has been involved in the military campaign against Houthi rebels in Yemen since March 2015.
Gause also noted Qatar's relationship with the Muslim Brotherhood "and other oppositional political groups", as well as Qatari cooperation with Iran on some issues "at a time when Saudi Arabia is confronting Iran directly on all sorts of fronts".
"Nobody wants to hurt Qatar. It has to choose whether it must move in one direction or another direction", he said.
The CNN report quoted the Qatari government communications office as saying it was working with the FBI and Britain's National Crime Agency on an ongoing hacking investigation.
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